Lysobacter pedocola sp nov., a novel species isolated from Korean soil

Abstract

A Gram-negative, yellow-pigmented bacterial strain, designated IPC6(T), was isolated from soil in an arid region of Goyang-si (Gyeonggi-do, South Korea). Cells were strictly aerobic, non-spore-forming, rod-shaped. The strain grew within a temperature range of 10-42A degrees C (optimum, 30A degrees C) and pH of 5.0-11.0 (optimum, pH 8.0) in the presence of 0-2% (w/v) NaCl. Phylogenetically, the novel strain was closely related to members of the Lysobacter genus based on 16S rRNA sequence similarity, and showed the highest sequence similarity to Lysobacter niastensis KACC 11588(T) (98.5%). The predominant fatty acids were iso-C-15:0, iso-C-16:0, and summed feature 9 (iso-C-17:1 omega 9c), with Q-8 identified as the major ubiquinone. The polar lipid content included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unknown aminophospholipid, and an unidentified phospholipid. DNA-DNA hybridization results indicated that the strain IPC6(T) was distinct from Lysobacter niastensis KACC 11588(T) (37.9 +/- 0.14%), Lysobacter panacisoli KACC 17502(T) (56.4 +/- 0.13%), Lysobacter soli KCTC 22011(T) (8.1 +/- 0.04%), Lysobacter gummosus KCTC 12132(T) (9.6 +/- 0.03%), and Lysobacter cavernae KCTC 42875(T) (37.5 +/- 0.14%), respectively. The DNA G + C content of the novel strain was 71.1 mol%. Based on the collective phenotypic, genotypic and chemotaxonomic data, the IPC6(T) strain is considered to represent a novel species in the genus Lysobacter, for which the name Lysobacter pedocola sp. nov. (= KCTC 42811(T) = JCM 31020(T)) is proposed.