Bioactivity guided isolation and structural characterization of the antidiabetic and antioxidant compound from bark extract of Avicennia officinalis L

Abstract

The present study was aimed at evaluating the antidiabetic activity of the ethanol bark extract of a mangrove plant Avicennia officinalis (AOEB) in streptozotocin (STZ)-induced diabetic mice. The study revealed AOEB could lower the blood glucose level significantly (p < .05) in diabetic mice at dose of 200 mg/kg b.w. The elevated total cholesterol, triglyceride, liver toxicity makers (SGOT and SGPT) and urea level were found to be ameliorated. The in vitro bioactivity-guided assay of AOEB led to isolation of a bioactive compound that inhibits the carbohydrate metabolizing enzymes (alpha-amylase and alpha-glucosidase) and also scavenging the DPPH, ABTS and superoxide radicals. The structure of the isolated compound was analyzed by different spectral techniques as FT-IR, CHNS, H-1 NMR, C-13 NMR, MS. Further, the isolated compound was analyzed by HPLC. However, further studies are required to elucidate the exact structure of the isolated compound. (C) 2019 SAAB. Published by Elsevier B.V. All rights reserved.