Lysobacter caseinilyticus, sp. nov., a casein hydrolyzing bacterium isolated from sea water

Abstract

A novel bacterial strain, designated KVB24(T), was isolated from sea-water of Busan Harbour in South Korea. Cells of strain KVB24(T) were Gram-stain negative, aerobic, rod shaped and non-motile. Strain KVB24(T) grew optimally at 25-28 degrees C and pH 6.5-7.0. Based on 16S rRNA gene sequence analysis, strain KVB24(T) was shown to belong to the genus Lysobacter within the class Gammaproteobacteria and to be closely related to Lysobacter dokdonensis DS-58(T), Lysobacter hankyongensis KTce-2(T) and Lysobacter niastensis GH41-7(T). DNA-DNA relatedness between strain KVB24(T) and its current closest relative was below 70%. The predominant fatty acids of strain KVB24(T) were iso-C-11:0, iso-C-11:0 3-OH, iso-C-14:0, iso-C-15:0, anteiso-C-15:0, iso-C-16:0 and summed feature 9 comprising (iso-C-17:1 omega 9c and/or 10 methyl C-16:0); the prominent isoprenoid was Q-8 and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The G + C content of genomic DNA from strain KVB24(T) was determined to be 67.5 mol%. Based on the phenotypic, genotypic and chemotaxonomic analyses, strain KVB24(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter caseinilyticus sp. nov. is proposed. The type strain is KVB24(T) (= KACC19816(T) = JCM32879(T)).