Nocardioides secundeburneus sp. nov. and Gordonia aurantiaca sp. nov., isolated from soil

Abstract

Two strains, B21T and C4-1T, were obtained from soil near Sinpung Station, the Republic of Korea. The two aerobic, Gramnegative, rod-shaped bacterial strains were designated B21T and C4-1T. Phylogenetic tree analysis based on 16S rRNA and whole-genome sequences suggested that the strains B21T and C4-1T belonged to the genera Gordonia and Nocardioides, respectively. The draft genome of strain B21ᵀ consists of 4.99 Mb with a G+C content of 68.3 mol%, while that of strain C4-1ᵀ is 4.80 Mb with a 72.8 mol% G+C content. The cells of strain B21T were non-motile and grew at a temperature of 15–50 °C (optimum, 40 °C), in the presence of 0–11.0% (w/v) sodium chloride (NaCl) (optimum, 0.0%) and at pH 5.0–12.0 (optimum, 8.0). The cells of strain C4-1T were non-motile and grew at a temperature of 15–30 °C (optimum, 30 °C), in the presence of 0–8.0% (w/v) NaCl (optimum, 0%) and at pH 5.0–10.0 (optimum, 8.0). The novel strains B21T and C4-1T exhibited average nucleotide identity values of 73.9–87.2% and 75.0–81.4% and digital DNA–DNA hybridization values of 19.9–32.8% and 20.0–24.0%, with reference strains in the genera Gordonia and Nocardioides, respectively. We thus propose the names Gordonia aurantiaca (type strain B21T=KACC 23739T=TBRC 19009T) and Nocardioides secundeburneus (type strain C4-1T=KACC 23738T=TBRC 19008T). © 2025 The Authors.