Cohnella terricola sp. nov., isolated from soil

Abstract

A Gram-positive, aerobic, flagellated, endospore-forming, rod-shaped strain, designated as G13(T), was isolated from soil. The results of 16S rRNA gene sequence analysis led to the conclusion that strain G13(T) was phylogenetically related to Cohnella boryungensis BR29(T) (97.5%) and Cohnella phaseoli CECT 7287(T) (96.9%) with digital DNA-DNA hybridization values of 21.0 and 21.4%, and distantly related to Cohnella thermotolerans CCUG 47242(T) (94.8%), type species of the genus Cohnella, at 19.0 %. The genome size of strain G13(T) was 5387258 bp, with 51.3 mol% G+C content. The predominant fatty acids were summed feature 9 (iso-C-17:1 omega 9c and/or C-16:0 10-methyl), anteiso-C-17:0, iso-C-17:0 and iso-C-15:0. The predominant quinone was menaquinone-7 and the major polar lipids were diphosphatidyglycerol, phosphatidylethanolamine, phosphatidylglycerol, lysylphosphatidylglycerol, three aminophospholipids, two phosphoglycolipids, three aminolipids and two unidentified lipids. Based on the data from phenotypic tests and the genotypic differences between strain G13(T) and its close phylogenetic relatives, strain G13(T) represents a new species belonging to the genus Cohnella, for which the name Cohnella terricola sp. nov. (=KACC 19905(T)=NBRC 113748(T)) is proposed.