Taibaiella lutea sp. nov., Isolated from Ubiquitous Weedy Grass

Abstract

An aerobic, Gram-stain-negative, non-motile and rod-shaped bacterial strain, designated as KVB11(T) was isolated from the root of plant Setaria viridis near Dongguk University. Phylogenetic analysis indicated that strain KVB11(T) belonged to the genus Taibaiella of the family Chitinophagaceae. Comparative 16S rRNA gene analysis indicated that the strain KVB11(T) has closest similarities to Taibaiella smilacinae PTJT-5( T) (96.6%) and Taibaiella yonginensis THG-SC4(T) (96.4%). Strain KVB11(T) grew optimally at 25-28 degrees C, at pH 7.0 and tolerates NaCl upto 1% (w/v) NaCl. Flexirubin-type pigments were present. Phylogenetic and phylogenomic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain KVB11(T) formed a distinct branch with all seven type strains of genus Taibaiella. MK-7 was identified as the predominant isoprenoid quinone. The polar lipid were phosphatidylethanolamine, one unidentified aminophosphoglycolipid, one unidentified aminoglycolipid and two unidentified phosphoglycolipids. C-15:0 (28.3%), iso-C-15:1 G (28.5%) and iso-C-17:0 3-OH (10.5%) were the major fatty acids of the strain KVB11(T). The DNA G + C content of the genomic DNA of novel isolate was determined to be 38.9%. The above results clearly confirmed that strain KVB11(T) represents a novel species of the genus Taibaiella, for which the name Taibaiella lutea sp. nov. is proposed. The type strain is KVB11(T) (= KACC 19904( T) = NBRC 113690( T)).