Anti-Inflammatory Effects of 6,8-Diprenyl-7,4 '-dihydroxyflavanone from Sophora tonkinensis on Lipopolysaccharide-Stimulated RAW 264.7 Cells

Abstract

The anti-inflammatory e alpha ects and molecular mechanism of 6,8-diprenyl-7,4'-dihydroxyflavanone (DDF), one of the flavanones found in Sophora tonkinensis, were assessed in vitro through macrophage-mediated inflammation in the present study. The anti-inflammatory effects of DDF were not previously reported. DDF inhibited the production of nitric oxide and the expression of tumor necrosis factor alpha, interleukin-1 beta, and interleukin-6. Furthermore, the activation of nuclear factor-kappa B (NF-kappa B) and extracellular signal-regulated kinases (ERKs) in lipopolysaccharide-stimulated macrophages was suppressed by treatment with DDF. Therefore, DDF demonstrated potentially anti-inflammatory effects via the blockade of NF-kappa B and ERK activation in macrophages.