Vibrio injenensis sp nov., isolated from human clinical specimens

Abstract

Vibrio species are well known as motile, mostly oxidase-positive, facultative anaerobic Gram-negative bacteria. They are abundant in aquatic environments and are a common cause of human infections including diarrhea, soft tissue diseases, and bacteremia. Here, two Gram-negative bacteria, designated M12-1144(T) and M12-1181, were isolated from human clinical specimens and identified using a polyphasic taxonomic approach. Phylogenetic study based on 16S rRNA gene sequence analysis revealed that the isolates belong to the genus Vibrio, and are closely related to Vibrio metschnikovii KCTC 32284(T) (98.3%) and Vibrio cincinnatiensis KCTC 2733(T) (97.8%). The major fatty acids were summed feature 3 (C-16:1 omega 7c/C-16:1 omega 6c, 38.0%), C-16:0 (23.0%), and summed feature 8 (C-18:1 omega 7c or C-18:1 omega 6c, 19.3%) and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The G + C content of the genomic DNA was determined to be 44.1 mol%. DNA-DNA relatedness between the two newly isolated strains and V. metschnikovii KCTC 32284(T) and V. cincinnatiensis KCTC 2733(T) was between 42.6 to 47.5%. The similarities of genome-to-genome distance between M12-1144(T) and related species ranged from 18.4-54.8%. Based on these results, a new species of the genus Vibrio, Vibrio injenensis is proposed. The type strain is M12-1144 (T)(=KCTC 32233(T) =JCM 30011(T)).