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Ex Vivo Surface Decoration of Phenylboronic Acid onto Natural Killer Cells for Sialic Acid-Mediated Versatile Cancer Cell Targetingopen access
- Issue Date
- Dec-2023
- Publisher
- American Chemical Society
- Keywords
- Benzeneboronic Acid; Lipid; N Acetylneuraminic Acid; Benzeneboronic Acid; Biocompatible Materials; Lipids; N-acetylneuraminic Acid; Carboxylic Acids; Cell Membranes; Cytology; Diseases; Fluorescence Spectroscopy; Acid Molecules; Cancer Cell Targeting; Cancer Cells; Ex-vivo; Mda-mb-231; Natural Killer Cells; Phenylboronic Acids; Phosphorylethanolamine; Sialic Acids; Surface Decoration; 1,2 Distearoyl Sn Glycero 3 Phosphorylethanolamine; Benzeneboronic Acid; Biomaterial; Fas Ligand; Lipid; Lipopolysaccharide; Sialic Acid; Tumor Necrosis Factor Related Apoptosis Inducing Ligand; Unclassified Drug; N Acetylneuraminic Acid; Antineoplastic Activity; Article; Binding Affinity; Biocompatibility; Cancer Cell; Cancer Diagnosis; Cell Surface; Cell Viability; Complex Formation; Controlled Study; Cytokine Release; Cytokine Response; Cytolysis; Ex Vivo Study; Fluorescence Intensity; Fluorescence Microscopy; Hct 116 Cell Line; Hep-g2 Cell Line; Human; Human Cell; Hydrophobicity; Immunocompetent Cell; In Vitro Study; Mda-mb-231 Cell Line; Natural Killer Cell; Ph; Spectrofluorometry; Surface Property; Synthesis; Target Cell; Tumor Spheroid; Chemistry; Metabolism; Neoplasm; Biocompatible Materials; Humans; Killer Cells, Natural; Lipids; N-acetylneuraminic Acid; Neoplasms
- Citation
- Biomacromolecules, v.25, no.1, pp 222 - 237
- Pages
- 16
- Indexed
- SCIE
SCOPUS
- Journal Title
- Biomacromolecules
- Volume
- 25
- Number
- 1
- Start Page
- 222
- End Page
- 237
- ISSN
- 1525-7797
1526-4602
- Abstract
- Phenylboronic acid (PBA) has been highly acknowledged as a significant cancer recognition moiety in sialic acid-overexpressing cancer cells. In this investigation, lipid-mediated biomaterial integrated PBA molecules onto the surface of natural killer (NK) cells to make a receptor-mediated immune cell therapeutic module. Therefore, a 1,2-distearoyl-sn-glycero-3-phosphorylethanolamine (DSPE) lipid-conjugated di-PEG-PBA (DSPEPEG-di(PEG-PBA) biomaterial was synthesized. The DSPEPEG-di(PEG-PBA) biomaterial exhibited a high affinity for sialic acid (SA), confirmed by fluorescence spectroscopy at pH 6.5 and 7.4. DSPEPEG-di(PEG-PBA) was successfully anchored onto NK cell surfaces (PBA-NK), and this biomaterial maintains intrinsic properties such as viability, ligand availability (FasL & TRAIL), and cytokine secretion response to LPS. The anticancer efficacy of PBA-NK cells was evaluated against 2D cancer cells (MDA-MB-231, HepG2, and HCT-116) and 3D tumor spheroids of MDA-MB-231 cells. PBA-NK cells exhibited greatly enhanced anticancer effects against SA-overexpressing cancer cells. Thus, PBA-NK cells represent a new anticancer strategy for cancer immunotherapy.
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Related Researcher
- Kim, Kyo Bum
- College of Engineering (Department of Chemical and Biochemical Engineering)