Lysobacter humi sp nov., isolated from soil

Abstract

A yellow-pigmented and strictly aerobic bacterial strain, designated FJY8(T), was isolated from the soil of Goyang, South Korea. The cells of FJY8(T) were Gram-reaction-negative, non-motile rods. Colonies were circular, convex and transparent. Strain FJY8(T) grew optimally at 30 degrees C, with 0% (w/v) NaCl and at pH 8. Phylogenetic analysis of the 16S rRNA gene sequence of FJY8(T) revealed a clear affiliation of this bacterium to the family Lysobacteraceae, and it was related to members of the genus Lysobacter, with Lysobacter xinjiangensis KCTC 22558(T) being its closest relative (98.7% sequence similarity). The DNA G+C content was 68.0 +/- 0.4 mol%. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were identified as the major polar lipids, and an unidentified phospholipid and two unidentified aminophospholipids were also detected as the minor polar lipids. The major fatty acids were iso-C-16: 0, summed feature 9 (iso-C-17 : (1)omega 9c and/or C-16 : 0 10-methyl) and iso-C-15 : 0. Only ubiquinone-8 (Q-8) was detected as the isoprenoid quinone. DNA-DNA hybridization values of strain FJY8(T) with Lysobacter xinjiangensis RCML-52(T) and Lysobacter mobilis 9NM-14(T) were 55.8 +/- 2.0 and 45.2 +/- 4.8 %, respectively. On the basis of DNA-DNA hybridization, phylogenetic distinctiveness, and some physiological and biochemical tests, strain FJY8(T) (=KCTC 42810(T) = JCM 31019(T)) represents a novel species of the genus Lysobacter, for which the name Lysobacter humi sp. nov. is proposed.