Isolation of Chemical Constituents from Athyrium yokoscense and Their Inhibitory Effects on Nitrite Accumulation in LPS-Stimulated BV2 Microglial Cells

초록

Three phloroglucinol derivatives, aspidinol B (1), aspidinol P (2), and methylene-bis-aspidinol B (3), together with one flavonoid, angophorol (4), were isolated from Athyrium yokoscense (Athyriaceae). The isolated compounds were evaluated for their effects on extracellular nitrite accumulation, an indirect index of nitric oxide production, in lipopolysaccharide-stimulated BV2 cells. The IC50 values for compounds 1, 2, and 3 were 34.8, 55.4, and 70.0 μM, respectively, whereas that for compound 4 exceeded 100 μM. Immunoblot analysis showed lower inducible nitric oxide synthase and cyclooxygenase-2 protein abundance after treatment with compound 1. Compound 2 reduced cyclooxygenase-2 abundance without a comparable reduction in inducible nitric oxide synthase. Compound 1 was associated with lower c-Jun N-terminal kinase phosphorylation at 100 and 200 μM and a pronounced reduction in extracellular signal-regulated kinase phosphorylation at 200 μM. p38 phosphorylation was not comparably suppressed. These findings identify aspidinol B and aspidinol P as constituents with inhibitory effects on nitrite accumulation and support further evaluation in primary or humanderived microglial models.

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