Isolation and characterization of Pseudarthrobacter cremeus sp. nov. and Ideonella flava sp. nov. from mountain soil

초록

Two novel bacterial strains, designated as YS3T and YS5T, were isolated from soil collected at Nogo Mountain, Gyeonggi-do, Republic of Korea. Strain YS3T is Gram-positive, aerobic, non-motile and rod-shaped, forming opaque, cream-coloured colonies with smooth surfaces. Phylogenetic analysis based on the 16S rRNA gene sequence revealed the highest similarity to Pseudarthrobacter humi RMG13T (99.2%) and Pseudarthrobacter siccitolerans 4J27T (99.2%). Strain YS3T grew at 4–35 °C (optimum, 30 °C), at pH 4.0–12.0 (optimum, pH 8.0), and tolerated up to 6% (w/v) NaCl, with optimal growth occurring in the absence of NaCl. The genomic G+C content of strain YS3T was 65.0 mol%. Average nucleotide identity (ANI) values between strain YS3T and related Pseudarthrobacter species ranged from 81.2% to 84.9%, while digital DNA–DNA hybridization (dDDH) values ranged from 20.7% to 39.0%. Strain YS5T is Gram-negative, aerobic, motile and rod-shaped, forming translucent, yellow colonies with smooth surfaces. Phylogenetic analysis based on the 16S rRNA gene sequence showed the highest similarity to Ideonella dechloratans CCUG 30898T (98.9%) and Ideonella alba 3Y2T (98.7%). Strain YS5T grew at 10–35 °C (optimum, 30 °C), at pH 4.0–12.0 (optimum, pH 8.0), and tolerated up to 1% (w/v) NaCl, with optimal growth in the absence of NaCl. The genomic G+C content of strain YS5T was 69.0 mol%. ANI values between strain YS5T and Ideonella species ranged from 77.9% to 81.2%, while dDDH values ranged from 15.1% to 22.4%. On the basis of phenotypic, genotypic, phylogenetic and chemotaxonomic evidence, strain YS3T represents a novel species of the genus Pseudarthrobacter, for which the name Pseudarthrobacter cremeus sp. nov. is proposed (type strain YS3T=KACC 24143T=TBRC 21184T). Similarly, strain YS5T represents a novel species of the genus Ideonella, for which the name Ideonella flava sp. nov. is proposed (type strain YS5T=KACC 24144T=TBRC 21186T). © 2026 The Authors.

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