ScholarWorks@동국대학교

초록

Microbial infections and colorectal cancer are significant contributors to mortality and morbidity, necessitating prompt action. In the present work, NiTiO3 (NT), curcumin-loaded NiTiO3 (CUNT), chitosan-loaded NiTiO3 (CHNT), and both curcumin and chitosan-loaded NiTiO3 (CUCHNT) hybrid nanomaterials (HNMs) were systematically prepared using Psidium guajava leaf extract. PXRD studies revealed that NT, CUNT, CHNT, and CUCHNT exhibited a rhombohedral structure with the R3 space group with mean crystallite sizes of similar to 66.41, similar to 61.94, similar to 61.65, and similar to 65.12 nm, respectively. Band gap energies were estimated as similar to 3.10, similar to 3.15, similar to 3.14, and similar to 3.15 eV for NT, CUNT, CHNT, and CUCHNT, respectively. Antibacterial efficacies of synthesized HNMs were analyzed against both Gram-positive (G+) and Gram-negative (G-) bacteria using the well diffusion method, and the zone of inhibition (ZOI) exhibited by NT, CUNT, CHNT, and CUCHNT against Staphylococcus aureus were 11.5, 13, 12, and 17.5 mm, respectively; those of Staphylococcus pneumoniae were 9, 13.5, 11, and 14 mm, respectively; those of Klebsiella pneumoniae were 10, 9.5, 10, and 17 mm, respectively; and those of Escherichia coli were 11, 11.5, 13, and 16.5 mm, respectively. ZOI results showed that CUCHNT HNMs have superior antibacterial efficacies for all tested bacterial strains compared to NT, CUNT, and CHNT. Cytotoxic capabilities of the synthesized HNMs were analyzed using the MTT assay against the HCT-116 colorectal carcinoma cell line. All HNMs showed significant anticancer activity, with CUCHNT exhibiting the most pronounced activity, having an IC50 value of 87 mu g/mL. Morphological changes and cell death mechanisms of synthesized HNMs were studied using dual AO/EtBr, H2DCFDA, and JC-1 staining. These findings emphasize that biosynthesized CUCHNT HNMs using P. guajava leaf extract could be a potential therapeutic agent for antimicrobial and anticancer applications.

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