ScholarWorks@동국대학교

초록

The objective of this study was to investigate the effect of brown and green macroalgae-derived bioactive compound compositions on enteric methane mitigation as a novel dietary intervention strategy. Response surface methodology (RSM) was employed to optimize the composition of various types of macroalgae (Ecklonia cava, Ecklonia stolonifera, and Ulva pertusa) for maximizing in vitro rumen methane abatement. Chemical compositions of each macroalgae were determined to either 0.50, 0.75, or 1 g for levels of Box-Behnken design (-1, 0, + 1, respectively) and they were mixed with rumen and fluid from the cattle's abomasum (total volume: 50 mL). From the in vitro experiment, the maximum methane abatement was up to 90.0 +/- 0.7%, when E. cava, E. stolonifera, and U. pertusa were mixed in a ratio of 1:1:0.75. The optimized compositions for maximum methane abatement, based on the prediction via total polyphenol and phloroglucinol contents, were estimated to 95.9% at 1:0.94:0.72 ratio. From the findings in this study, the optimized macroalgae compositions of livestock feed can contribute an eco-friendly method for abatement of enteric methane as mitigating the ruminant animals on global greenhouse gas (GHG) emissions.Graphical AbstractIn vitro experimental procedure for methane gas measurement

키워드