Photo-triggered fluorescent labelling of recombinant proteins in live cells

Jung, Deokho; Sato, Kohei; Min, Kyoungmi; Shigenaga, Akira; Jung, Juyeon; Otaka, Akira; Kwon, Youngeun

doi:10.1039/c5cc01067e

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Photo-triggered fluorescent labelling of recombinant proteins in live cells

Jung, Deokho;
Sato, Kohei;
Min, Kyoungmi;
Shigenaga, Akira;
Jung, Juyeon;
... Kwon, Youngeun;
외 1명

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초록

A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.

키워드

SPLIT-INTEIN; FLUOROGENIC PROBES; PEPTIDE CARRIER; DNAE INTEIN; IN-VIVO; IMMOBILIZATION; ACTIVATION; LIGATION; CLEAVAGE; DELIVERY

제목: Photo-triggered fluorescent labelling of recombinant proteins in live cells

저자: Jung, Deokho; Sato, Kohei; Min, Kyoungmi; Shigenaga, Akira; Jung, Juyeon; Otaka, Akira; Kwon, Youngeun

DOI: 10.1039/c5cc01067e

발행일: 2015-05

유형: Article

저널명: Chemical Communications

권: 51

호: 47

페이지: 9670 ~ 9673

언어: ENG

출판사: ROYAL SOC CHEMISTRY

발행국가: 영국

분량: 4 페이지

ISSN: E 1364-548X
P 1359-7345