Characterization of Pedobacter nemoris sp. nov. isolated from park soil

초록

A facultatively anaerobic and Gram-negative, catalase- andoxidase-positive, rod-shaped, non-motile, and pink-pigmentedstrain designated GSP4T was isolated from park soil. Theoptimal growth conditions for strain GSP4T are R2A agar, pH7.0, 0% NaCl (w/v), and 30°C. Phylogenetic analysis based on16S rRNA gene sequences revealed that strain GSP4T belongsto the genus Pedobacter. Strain GSP4T indicated the highest16S rRNA sequence similarity with Pedobacter kyonggii K-4-11-1T (98.82%), Pedobacter suwonensis 15-52T (98.26%), andPedobacter terrae DS-57T (98.20%). The DNA G + C contentof strain GSP4T was 40.3 mol%. The average nucleotideidentity and digital DNA-DNA hybridization values betweenstrain GSP4T and strains belonging to the genus Pedobacterranged from 76% to 83% and 13% to 30%, respectively. Themajor fatty acids were iso-C15:0, iso-C17:0 3-OH and summedfeature 3. The polar lipid profile consists of one phosphatidylglycerol,two glycolipids, two aminoglycolipids, and oneaminolipid. Based on phylogenomic, phenotypic, physiological,and chemotaxonomic characteristics, strain GSP4T is proposedas a novel species of the genus Pedobacter, for which the namePedobacter nemoris sp. nov. is proposed (type strain = KACC24141ᵀ = TBRC 21189ᵀ).

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