상세 보기
- Song, J.;
- Kim, D.;
- Jung, J.;
- Choi, E.;
- Lee, Y.;
- ... Joo, J.W.J.;
- ... Jang, W.;
- 외 8명
WEB OF SCIENCE
1SCOPUS
1초록
Colorectal cancer (CRC) is one of the top five most common and life-threatening malignancies worldwide. Most CRC develops from advanced colorectal adenoma (ACA), a precancerous stage, through the adenoma-carcinoma sequence. However, its underlying mechanisms, including how the tumor microenvironment changes, remain elusive. Therefore, we conducted an integrative analysis comparing RNA-seq data collected from 40 ACA patients who visited Dongguk University Ilsan Hospital with normal adjacent colons and tumor samples from 18 CRC patients collected from a public database. Differential expression analysis identified 21 and 79 sequentially up- or down-regulated genes across the continuum, respectively. The functional centrality of the continuum genes was assessed through network analysis, identifying 11 up- and 13 down-regulated hub-genes. Subsequently, we validated the prognostic effects of hub-genes using the Kaplan–Meier survival analysis. To estimate the immunological transition of the adenoma-carcinoma sequence, single-cell deconvolution and immune repertoire analyses were conducted. Significant composition changes for innate immunity cells and decreased plasma B-cells with immunoglobulin diversity were observed, along with distinctive immunoglobulin recombination patterns. Taken together, we believe our findings suggest underlying transcriptional and immunological changes during the adenoma-carcinoma sequence, contributing to the further development of pre-diagnostic markers for CRC. © The Author(s) 2024.
키워드
- 제목
- Elucidating immunological characteristics of the adenoma-carcinoma sequence in colorectal cancer patients in South Korea using a bioinformatics approach
- 저자
- Song, J.; Kim, D.; Jung, J.; Choi, E.; Lee, Y.; Jeong, Y.; Lee, B.; Lee, S.; Shim, Y.; Won, Y.; Cho, H.; Jang, D.K.; Kang, H.W.; Joo, J.W.J.; Jang, W.
- 발행일
- 2024-05
- 유형
- Article
- 권
- 14
- 호
- 1