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Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase
- Cho, Jin-Beom;
- Lee, Jin-Moo;
- Ahn, Hee-Chul;
- Jeong, Yong-Joo
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21초록
A new chemical inhibitor against severe acute respiratory syndrome (SARS) coronavirus helicase, 7-ethyl-8-mercapto-3-methyl-3,7-dihydro-1H-purine-2,6-dione, was identified. We investigated the inhibitory effect of the compound by conducting colorimetry-based ATP hydrolysis assay and fluorescence resonance energy transfer-based double-stranded DNA unwinding assay. The compound suppressed both ATP hydrolysis and double-stranded DNA unwinding activities of helicase with IC50 values of 8.66 +/- 0.26 mu M and 41.6 +/- 2.3 mu M, respectively. Moreover, we observed that the compound did not show cytotoxicity up to 80 mu M concentration. Our results suggest that the compound might serve as a SARS coronavirus inhibitor.
키워드
SARS; helicase; ATP hydrolysis; dsDNA unwinding; inhibitor; ACUTE RESPIRATORY SYNDROME; REPLICATION INHIBITOR; NTPASE/HELICASE; HEPATITIS
- 제목
- Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase
- 저자
- Cho, Jin-Beom; Lee, Jin-Moo; Ahn, Hee-Chul; Jeong, Yong-Joo
- 발행일
- 2015-12
- 유형
- Article
- 권
- 25
- 호
- 12
- 페이지
- 2007 ~ 2010