Efficient degradation and detoxification of methylene blue dye by a newly isolated ligninolytic enzyme producing bacterium Bacillus albus MW407057

초록

In present work, a LiP enzyme producing bacterium was isolated form textile wastewater and sludge sample and identified as Bacillus albus by 16S rRNA gene sequencing analysis. This bacterium decolorized 99.27 % MB dye and removed 83.87 % COD within 6 h at 30 degrees C, pH 7, 100 rpm and 100 mg/l of dye concentration in presence of glucose and yeast extract as carbon and nitrogen source, respectively. The bacterium also produced LiP enzyme of molecular weight -48 kDa, characterized by SDS-PAGE analysis. Different metabolites like monomethylthionine, thionin, (E)-2-(3-Oxopropylidene)-2H-benzo[b][1,4] thiozine-3-carboxylic acid, N-(3,4-dihydroxyphenyl)-N-methylformamide, ethylamine, water and carbon dioxide produced during treatment process were characterized by FT-IR and LC-MS analysis. Further, the toxicity assessment results showed that the toxicity of bacteria treated dye solution was reduced significantly allowing 90 % seed germination indicating that the isolated bacterium B. albus has high potential to decolorize and detoxify MB dye for environmental safety.

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