Paenibacillus mobilis sp nov., a Gram-stain-negative bacterium isolated from soil

Abstract

A novel Gram-stain-negative bacterium, designated strain S8(T), was isolated from a soil sample obtained in Gyeonggi Province, Republic of Korea. Cells of strain S8(T) were endospore-forming, motile by means of peritrichous flagella, and rod-shaped. S8(T) colonies were round, convex, wavy and white. Strain S8(T) grew optimally at 37 degrees C, pH 6-8, and up to 2.0% (w/v) NaCl. Based on 16S rRNA gene sequence similarity, strain S8(T) was affiliated with the genus Paenibacillus in the family Paenibacillaceae and was most closely related to Paenibacillus yonginensis DCY84(T) and Paenibacillus physcomitrellae XBT (98.8 and 97.1% sequence similarity). The DNA G+C content of the novel strain was 53.1 +/- 0.3 mol%. Strain S8(T) contained diphosphatidylglycerol, phosphatidylglycerol, two phospholipids, four aminophospholipids, an aminolipid and three unidentified lipids. The major fatty acid was anteiso-branched C-15 : 0 The quinone was menaquinone MK-7. The peptidoglycan of strain S8(T) contained mesodiaminopimelic acid. The DNA-DNA hybridization values of strain S8(T) with P. yonginensis KCTC 33428(T) and P. physcomitrellae DSM 29851(T) were 44% and 32 %, respectively. Data from the DNA-DNA hybridization, biochemical, phylogenetic and physiological analyses indicate that strain S8(T) (= KCTC 33848(T) = JCM 31672(T)) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus mobilis sp. nov. is proposed.