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Whole genome re-sequencing and development of SSR markers in oriental melonopen access

Authors
Song, W.-H.Chung, S.-M.
Issue Date
2019
Publisher
Korean Society of Plant Biotechnology
Keywords
Indel; Oriental melon; Polymorphic markers; Resequencing; SSR
Citation
Journal of Plant Biotechnology, v.46, no.2, pp 71 - 78
Pages
8
Indexed
SCOPUS
KCI
Journal Title
Journal of Plant Biotechnology
Volume
46
Number
2
Start Page
71
End Page
78
URI
https://scholarworks.dongguk.edu/handle/sw.dongguk/8555
DOI
10.5010/JPB.2019.46.2.071
ISSN
1229-2818
2384-1397
Abstract
The objective of this study was to use ‘Danta PR’, NGS (Next Generation Sequencing) technology for genome resequencing to develop polymorphic makers between Chinese oriental melon, ‘Hyangseo 1’ and Korean oriental melon. From the resequencing data that covered about 81 times of the genome size, 104,357 of SSR motifs and Indel, and 1,092,436 of SNPs were identified. 299 SSR and 307 Indel markers were chosen to cover each chromosome with 25 markers. These markers were subsequently used to identify genotypes of ‘Danta PR’ BC1 (F1 x ‘Danta PR’) population and a genetic linkage map was constructed. SSR, Indel, and SNPs identified in this study would be useful as a breeding tool to develop new oriental melon varieties. © 2019 Korean Society of Plant Biotechnology. All rights reserved.
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