Priming via three-dimensional culture in hydrogels accelerates retroviral gene transduction of human mesenchymal stem cells

Abstract

Statement of Purpose: Numerous studies demonstrated that environmental dimensionality plays an important role in cell behavior.1,2 Cells cultured in three-dimensional (3D) environments markedly differ from those cultured on conventional two-dimensional (2D) tissue culture plates. There are numerous approaches to improve the low transduction efficiency of retroviral vectors.4-6 However, most studies investigating whether cell cycle regulation enhances retroviral gene transduction have been performed in 2D environments, and the effect of a 3D microenvironment, which better mimics in vivo conditions, is unknown. Cytocompatible hyaluronic acid (HA) hydrogels are a good candidate to study this issue. In this study, we are reporting a novel priming method for efficient and rapid retroviral gene transduction using photocrosslinkable HA hydrogels. © 2019 Omnipress - All rights reserved.