Flavobacterium edaphi sp. nov., isolated from soil from Jeju Island, Korea

Abstract

An aerobic, Gram-stain-negative, bright yellow-pigmented, oxidase and catalase-positive, non-motile, non-spore forming, rod-shaped strain designated DMN11(T) was isolated from the soil of crossroads of Jeju Island in South Korea. Colonies were circular, bright yellow-pigmented and smooth with regular edges and measured approximately 1-2mm in diameter. Flexirubin-type pigments were absent. Phylogenetic tree analysis based on the 16SrRNA gene sequence revealed that the strain DMN11(T) formed a lineage within the family Flavobacteriaceae of the phylum Bacteroidetes, and it was the most closely related to Flavobacterium suzhouense XIN-1(T) and Flavobacterium hauense BX12(T) (98.6% and 98.2% similarity, respectively). The major isoprenoid quinone was MK-6. The major fatty acids were summed feature 3 (comprising C(16:1)7c and/or C(16:1)6c), iso-C-15:0 and iso-C-15:0 3OH. The polar lipid profile of the strain DMN11(T) showed the presence of phosphatidylethanolamine (PE) as major lipid. The DNA G+C content was 35.3mol%, as determined by the thermal denaturation method. The mean levels of DNA-DNA relatedness of the strain DMN11(T) with F. suzhouense XIN-1(T) and F. hauense BX12(T) were 20.5% and 29.2%, respectively. Thus, the data accumulated in this study support the suggestion that the strain DMN11(T) is considered to represent a novel species of the genus Flavobacterieum, for which the name Flavobacterium edaphi sp. nov. is proposed. The type strain is DMN11(T) (=KCTC 62114(T)=JCM 32372(T)).