Runella soli sp. nov., isolated from garden soil

Abstract

A Gram-stain negative, aerobic, salmon-pink, non-motile and rod-shaped bacterium, designated strain 15J11-1(T), was isolated from a soil sample collected from the university garden in Nowongu, South Korea. The 16S rRNA gene sequence analysis showed that strain 15J11-1(T) is phylogenetically related to Runella slithyformis DSM 19594(T) and Runella palustris HMF3829(T) (96.9% and 95.4% sequence similarity, respectively). The major fatty acids of strain 15J11-1(T) were identified as iso-C-15:0, iso-C-17:0 3-OH, C-16:1 omega 5c and summed feature 3 (C-16:1 omega 7c and/or C-16:1 omega 6c). The predominant respiratory quinone was identified as MK-7. The polar lipids were found to comprise of phosphatidylethanolamine, three unidentified aminolipids, five unidentified glycolipids, two unidentified aminoglycolipids, an unidentified phospholipid and an unidentified polar lipid. The G+C content in the genomic DNA of the strain 15J11-1(T) was determined to be 49.9mol%. Based on the results of genotypic, phenotypic and chemotaxonomic analyses, strain 15J11-1(T) is concluded to represent a novel species of the genus Runella, for which the name Runella soli sp. nov. (type strain 15J11-1(T)=KCTC 52021(T)=NBRC 112817(T)) is proposed.