Ilyomonas limi gen. nov., sp. nov., a new member of the family Chitinophagaceae isolated from mud

Abstract

A Gram-strain negative, aerobic, catalase and oxidase positive, non-motile, short rod-shaped bacterium, designated 17mud1-8(T), was isolated from mud collected from Nowon-gu, Seoul, South Korea. The strain was found to be able to grow at 10-40 degrees C (optimum 28-30 degrees C), pH 5.0-8.0 (optimum 7.0), and in the absence of NaCl. The nearly full-length 16S rRNA gene of strain 17mud1-8(T) exhibits sequence similarity of 94.1% with that of Panacibacter ginsenosidivorans Gsoil 1550(T), followed by 93.6% sequence similarity with Parafilimonas terrae DSM 28286(T). Phylogenetic analysis indicated that strain 17mud1-8(T) belongs to the family Chitinophagaceae, sharing approximately 94.1-91.9% sequence similarity with members of closely related genera. The respiratory quinone was identified as MK-7. The predominant fatty acids were found to consist of iso-C-15:0, iso-C-17:1 omega 5c and iso-C-15:1 G. The polar lipids were identified as phosphatidylethanolamine, an unidentified aminophospholipid, an unidentified glycolipid, ten unidentified aminolipids and seven unidentified lipids. The draft genome of 17mud1-8(T) has G+C content of 40.9 mol% and a 5.8 Mb chromosome. On the basis of the phenotypic and genotypic properties, and phylogenetic inference, strain 17mud1-8(T) was found to represent a novel genus in the family Chitinophagaceae, for which the name Ilyomonas limi gen. nov., sp. nov. is proposed, with the type strain 17mud1-8(T)(=KCTC 52874(T) = NBRC 112826(T)).