Hymenobacter setariae sp. nov., isolated from the ubiquitous weedy grass Setaria viridis

Abstract

A Gram-stain-negative, short-rod, aerobic, non-motile, red to pink-pigmented bacterium, designated Fur1(T), was isolated from the dry spikelet clusters of a plant called Setaria viridis near Dongguk University. Phylogenetic analysis conducted based on 16S rRNA gene sequences indicated that strain Fur1(T) belonged to the genus Hymenobacter of the family Hymenobacteraceae. The 16S rRNA gene of Fur1(T) showed highest sequence similarity to those of Hymenobacter metalli KACC 17381(T) (97.5 %) and Hymenobacter marinus KACC 19042(T) (97.1 %). Growth occurred at 4-37 degrees C (optimum, 25-28 degrees C), up to 1.0 % NaCl (optimum, 0 %) and pH 5.5-9.0 (optimum, pH 6.0-7.5). The major fatty acids of strain Fur1(T) were identified as iso-C-15:0, C-16:1 omega 5c, anteiso-C-15:0, summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c) and summed feature 4 (comprising anteiso-C17:1B and/or iso-C17:1I) as the major cellular fatty acids. The predominant respiratory quinone was identified as MK-7. The polar lipids were phosphatidy-lethanolamine, five unidentified aminophospholipids, two unidentified phospholipids, one unidentified glycolipid and one uni-dentified polar lipid. The genomic DNA G+C content based on the draft genome sequence was 58.7 mol%. DNA-DNA relatedness between strain Fur1(T) and its closest relative was below 70 %. Characterization based on phylogenetic, chemotaxonomic and phenotypic analyses clearly indicated that strain Fur1(T) represents a novel species of the genus Hymenobacter, for which the name Hymenobacter setariae sp. nov. is proposed. The type strain is Fur1(T) (=KACC 19903(T)=NBRC=113691(T)).