Development of Co-hemin MOF/chitosan composite based biosensor for rapid detection of lactose

Abstract

An electrochemical biosensor containing Co-hemin metal organic frameworks (Co-hemin MOF)/chitosan composite and cellobiose dehydrogenase (CDH) was developed for lactose dectection. The formation of the Co-hemin MOF was verified by scanning electron microscopy, Fourier transform infrared spectroscopy, UV-vis absorption spectroscopy and electrochemical techniques. The Co-hemin MOF/chitosan composite exhibited outstanding electrochemical performance on lactose detection with high sensitivity (102.3 mu A mM(-1) cm(-2)) and fast response (5 s). More importantly, the lower determination limit of 4 mM and the broad linear ranges of 10 to 100 mM were obtained to detect lactose. With broad linear ranges, the feasibility of dairy application of the biosensor based on Co-hemin MOF electrode was demonstrated in this study. (C) 2020 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.