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Photo-triggered fluorescent labelling of recombinant proteins in live cells
- Authors
- Jung, Deokho; Sato, Kohei; Min, Kyoungmi; Shigenaga, Akira; Jung, Juyeon; Otaka, Akira; Kwon, Youngeun
- Issue Date
- May-2015
- Publisher
- ROYAL SOC CHEMISTRY
- Citation
- CHEMICAL COMMUNICATIONS, v.51, no.47, pp 9670 - 9673
- Pages
- 4
- Indexed
- SCI
SCIE
SCOPUS
- Journal Title
- CHEMICAL COMMUNICATIONS
- Volume
- 51
- Number
- 47
- Start Page
- 9670
- End Page
- 9673
- ISSN
- 1359-7345
1364-548X
- Abstract
- A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.
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Related Researcher
- Kwon, Young Eun
- College of Life Science and Biotechnology (Department of Biomedical Engineering)