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Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicaseopen access
- Issue Date
- Dec-2015
- Publisher
- KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
- Keywords
- SARS; helicase; ATP hydrolysis; dsDNA unwinding; inhibitor
- Citation
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.25, no.12, pp 2007 - 2010
- Pages
- 4
- Indexed
- SCIE
SCOPUS
KCI
- Journal Title
- JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
- Volume
- 25
- Number
- 12
- Start Page
- 2007
- End Page
- 2010
- ISSN
- 1017-7825
1738-8872
- Abstract
- A new chemical inhibitor against severe acute respiratory syndrome (SARS) coronavirus helicase, 7-ethyl-8-mercapto-3-methyl-3,7-dihydro-1H-purine-2,6-dione, was identified. We investigated the inhibitory effect of the compound by conducting colorimetry-based ATP hydrolysis assay and fluorescence resonance energy transfer-based double-stranded DNA unwinding assay. The compound suppressed both ATP hydrolysis and double-stranded DNA unwinding activities of helicase with IC50 values of 8.66 +/- 0.26 mu M and 41.6 +/- 2.3 mu M, respectively. Moreover, we observed that the compound did not show cytotoxicity up to 80 mu M concentration. Our results suggest that the compound might serve as a SARS coronavirus inhibitor.
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Related Researcher
- Ahn, Hee Chul
- College of Pharmacy (Department of Pharmacy)