Ensifer collicola sp nov., a bacterium isolated from soil in South Korea

Abstract

Strain Mol12(T), which presented in the form of Gram-negative, motile, non-spore forming rod-shaped, was isolated from soil in South Korea and characterized to determine its taxonomic position. The strain grew at 20-30A degrees C (optimum 30A degrees C) and pH 7.0-10.0 (optimum pH 8.0) with 1% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain Mol12(T) was most closely related to Ensifer terangae LMG 7834(T) (96.78%), Rhizobium daejeonense KCTC 12121(T) (96.43%), Ensifer adhaerens Casida A(T) (96.28%). Chemotaxonomic data showed that the predominant fatty acids were Summed Feature 8 (C-18:1 omega 7c and/or C-18:1 omega 6c; 53.02%) and C-18:1 omega 7c 11-methyl (24.01%). Its complex polar lipid contained major amounts of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE) and Q-10 as the predominant ubiquinone. The DNA G+C content of strain Mol12(T) was determined to be 60.9 mol%. Based on the phylogenetic, chemotaxonomic, and phenotypic data, strain Mol12(T) (=KCTC 42816(T) =JCM 31049(T)) ought to be classified as a type strain of a novel species, for which the name Ensifer collicola sp. nov. is proposed.