Draft Genome Sequence Analyses of Two Novel Marinobacter suadae sp. nov. and Wenyingzhuangia gilva sp. nov. Isolated from the Root of Suaeda japonica Makino

Abstract

Gram-negative, rod-shaped, and aerobic bacteria designated chi1(T) and chi5(T) were isolated from the root of Suaeda japonica Makino. Phylogenetics utilizing 16S rRNA and whole-genome sequences of the two novel strains chi1(T) and chi5(T) confirmed that they were related to the genera Marinobacter and Wenyingzhuangia, respectively. For the novel strains chi1(T) and chi5(T), the digital DNA-DNA hybridization values (19-20% and 22.1-36.6%, respectively) and average nucleotide identity values (74.4-76.5% and 79.1-88.9%, respectively) fell within the range for the genera Marinobacter and Wenyingzhuangia, respectively. Pangenome analyses of the novel strains chi1(T) and chi5(T) revealed 357 and 368 singletons genes, respectively. The genomic DNA G + C contents of the strains chi1(T) and chi5(T) were 57.2% and 31.5%, respectively. The major fatty acids of strain chi1(T) were C-12:0, C-16:0, and summed feature 3 (C-16:1 omega 6c and/or C-16:1 omega 7c), while those of the strain chi5(T) were iso-C-15:0 3OH, iso-C-17:0 3OH, and iso-C-15:0. Data from the phylogenetic, phylogenomic, pangenome, genomic, physiological, and biochemical analyses indicated that the novel strains were distinct. Therefore, we propose the names Marinobacter suadae (type strain chi1(T) = KACC 23259(T) = TBRC 17652(T)) and Wenyingzhangia gilva (type strain chi5(T) = KACC 23262(T) = TBRC 17900(T)) for the studied bacterial strains.