Synthesis of SnO2-Sodium alginate-polyethylene glycol-crocin nanocomposite for enhanced antimicrobial and anticancer activity

Abstract

Bacteria and fungi contribute to antibiotic resistance, posing a growing threat to human health, while cervical cancer remains a significant health concern, impacting women's well-being and mortality rates worldwide. In the present work, the efficiency of formulated tin oxide-sodium alginate-polyethylene glycol-crocin nanocomposites (SnO2-SAPEG-Cro-NCs) on antimicrobial as well as growth inhibition and apoptosis induction in cervical cancer cells was evaluated. The developed nanostructure was exposed to several spectroscopic investigations, including UV, FTIR, XRD, PL, EDAX, DLS studies, and FESEM structural observation. The results of the antimicrobial study revealed increased inhibition zones from 8.5 to 16 mm around the SnO2-SAPEG-Cro-NCs treatment over different pathogens, which proves its antimicrobial efficacy. The findings of the MTT study demonstrated that SnO2-SAPEG-Cro-NCs (0.5–10 μg/mL) treatment remarkably inhibited the HeLa cell viability with IC50 concentration of 5 μg/mL. The SnO2-SAPEG-Cro-NCs-treated HeLa cells also revealed increased intracellular ROS generation at 5 and 7.5 μg/mL, which may facilitate oxidative stress-mediated cell death. Further, the dual staining and DAPI staining results demonstrated that the SnO2-SAPEG-Cro-NCs treatment effectively increased the apoptosis in the cervical cancer HeLa cells. Overall, the outcomes authorized that SnO2-SAPEG-Cro-NCs is an excellent antimicrobial and anticancer agent for future use. © 2024 Elsevier B.V.