Roseateles albus sp. nov., Roseateles koreensis sp. nov. and Janthinobacterium fluminis sp. nov., isolated from freshwater at Jucheon River, and emended description of Roseateles aquaticus comb. nov.

Abstract

Three Gram-stain-negative, facultatively anaerobic, rod-shaped, catalase-positive, oxidase-negative bacterial strains were designated as hw1T, hw8T and hw3T. Strains hw1T, hw8T and hw3T grew at 15–28°C (optimum, 25°C), 15–35°C (optimum, 30°C) and 4–28°C (optimum, 20°C), respectively, and at pH 7.0–12.0 (optimum, pH 9.0), pH 6.0–11.0 (optimum, pH 9.0) and 5.0–12.0 (optimum, pH 7.0), respectively. Additionally, strains hw1T and hw8T only grew when the NaCl concentration was 0%, while strain hw3T grew at between 0 and 0.5% (w/v; optimum, 0%). The average nucleotide identity (ANI) values between strains hw1T, hw8T and the Roseateles type strains ranged from 73.8 to 84.2%, while the digital DNA–DNA hybridization (dDDH) values ranged from 19.7 to 27.5%. The ANI values between strain hw3T and the Janthinobacterium type strains ranged from 78.7 to 80.7%, while dDDH values ranged from 22.3 to 23.0%. The draft genomes of strains hw1T, hw8T and hw3T consisted of 5.5, 4.4 and 5.9 Mbp, with DNA G+C contents of 61.7, 61.8 and 66.0mol%, respectively. The results of the dDDH, ANI, phylogenetic, biochemical and physiological analyses indicated that the novel strains were distinct from other members of their genera. Thus, we proposed the names Roseateles albus sp. nov. (type strain hw1T= KACC 22887T= TBRC 16613T), Roseateles koreensis sp. nov. (type strain hw8T= KACC 22885T= TBRC 16614T) and Janthinobacterium fluminis sp. nov. (type strain hw3T= KACC 22886T= TBRC 16615T). © 2023 The Authors.