RAW 264.7 세포에서 Metformin과 병행투여 시 상승효과를 나타내는 한약재의 선별 연구

Abstract

Objectives: This study was performed to evaluate the effects of Metformin and Lonicerae Flos,Agrobacterium Rhizogenes, Coptidis Rhizoma, Atractylodis Rhizoma Alba, Houttuyniae Herbaextracs combinations on hypoglycemia in RAW 264.7 cells. Methods: Expressions of Sirt1, p-adenosine monophosphate-activated kinase (p-AMPK), AMPKalpha,peroxisome proliferator activated receptor (PPAR)-alpha, PPAR-gamma, X-box bindingprotein 1 (XBP-1), tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 were analyzed by realtime polymerase chain reaction and Western blotting analysis. Results: The level of gene expression of Sirt1, p-AMPK, AMPK-alpha, PPAR-alpha and XBP-1 inrelation to that of beta-actin were increased or decreased significantly with the Metformin andLonicerae Flos, Agrobacterium Rhizogenes extracts combination groups. The level of geneexpression of TNF-alpha and IL-6 were increased significantly with the Metformin andHouttuyniae Herba, Coptidis Rhizoma extracts combination groups. Conclusions: Metformin and Lonicerae Flos, Agrobacterium Rhizogenes extracts combinationgroups showed synergistic hypoglycemic effects by increasing AMPK and PPAR gene expressionin RAW 264.7 cells