Characterization of enzymatic saccharification for lignocellulosic biomass pretreated with peracetic acid

Abstract

In this study, the lignocellulosic fibers from Quercus mongolica and Larix kaempferi wood chips pretreated with peracetic acid were enzymatically hydrolyzed by CTec2 and HTec2, and the characteristics of enzymatic saccharification were compared and analyzed with the difference of chemical composition of biomass. The pretreated wood fibers of two species could be hydrolyzed only with CTec2. The optimum CTec2 dosage for wood fibers was different depending on the species of the biomass (Quercus mongolica'. ∼19 FPU/g biomass, Larix kaempferi'. ∼21 FPU/g biomass), however, it could be economized by using together with endoxylanase, HTec2. The synergy effect of hydrolysis reaction was showed in the saccharification by using both of enzymes, and the most effective mixing condition of enzymes was dependent on biomass (CTec2:HTec2; 7:3 in Quercus mongolica, 9:1 in Larix kaempferi). The hydrolysis of xylan has been activated by endoxylanase, therefore, it could saved the CTec2 dosage and economized enzymatic saccharification of wood fibers, especially in the case of Quercus mongolica.